Tiangen biotech china
Webb13 apr. 2024 · The reverse transcription reaction with 1 μg of total RNA was carried out with a cDNA synthesis kit (KR116, Tiangen Biotech Co., LTD, Beijing, China). Thereafter, PCR amplification was performed using SYBR Green PCR Master Mix (208054, Qiagen) and Roche LightCycler® 960 system under the following condition: ... Webbför 2 dagar sedan · The fecal DNA was isolated using TIANGEN stool DNA kit (Beijing, China) according to the manufacturer’s recommendations. ... followed by a high-pressure cell crusher (Union-Biotech co LTD). The supernatants were collected, run through a Ni-NTA agarose resin (Qiagen), and washed with 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, …
Tiangen biotech china
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Webb7 apr. 2024 · Total RNA was extracted by a Quick RNA isolation kit (Tiangen Biotech, Beijing, China), and the RNA integrity number (RIN) was measure by an Agilent 2100 bioanalyzer (Santa Clara, CA, USA) and characterized on 1 % agarose gels using a spectrophotometer (NanoDrop Technologies Co., Ltd., Wilmington, USA). WebbDNA was extracted using a mass plasmid extraction kit (Qiagen, Shanghai, China). The concentration and purity were then calculated according to the OD260/OD280 ratio, and the DNA was then preserved in 20°C,RT-PCR was then conducted, using a FastQuant RT Kit (TIANGEN BIOTECH) according to the manufacturer’s instructions.
Webb28 apr. 2016 · The resulting PCR products were cloned using a ZeroBack Fast Ligation Kit (Tiangen Biotech, Beijing, China) and sequenced via conventional Sanger dideoxy sequencing (Perkin Elmer, Wellesley, MA, USA). The DNAStar Lasergene package (Version 7.1.0, DNAStar Inc., Madison, WI, USA) was used for the full-length genome assembly of … http://www.sell147.com/company/China-Mainland/181122.htm
Webb22 juli 2024 · TIANGEN BIOTECH ( BEIJING) CO., LTD. is a high-tech biological enterprise integrating R&D, production, sales, and customer service. It has been committed to … http://sv.tiangenbioem.com/product-categories/
Webbför 15 timmar sedan · Total RNA was extracted with RNAprep Pure Plant Plus Kit (TIANGEN Biotech, Beijing, China). First strand cDNA was synthesized with FastKing gDNA Dispelling RT SuperMix (TIANGEN Biotech, Beijing, China). RT-qPCR analysis was performed with the QuantStudio 6 (Thermo Fisher).
WebbThe 20 μL PCR mixture contained 50–150 ng of genomic DNA and 10 μL 2× PCR mix (Tiangen Biotech). For PCR amplification, an initial denaturation at 95°C for 5 minutes was performed followed by 36 cycles at 95°C for 30 seconds, 64°C for 50 seconds, 72°C for 20 seconds, and a final extension at 72°C for 10 minutes. kent offers to take lear to a brotherWebbTIANGEN Biotech. Manufacturing · China · 58 Employees . TIANGEN BIOTECH is gathering R&D, manufacture, distribution and customer service as integral whole in life-science … kent office trailersWebbTIANGEN (BEIJING) BIOTECH mission is to supply the customized biological reagent and professional technical services. On basis of the abundant capital, advanced … ken toda high point ncWebbBacterial Genomic Dna Extraction Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, … kent october half term dates 2022Webb30 nov. 2024 · WASHINGTON, Nov. 30, 2024 /PRNewswire/ -- TIANGEN, a leading brand in the field of nucleic acid purification in China, has announced its participation in the Cell … is indeed only for usWebb7 apr. 2024 · Genomic DNA was extracted from peripheral blood leukocytes by using TIANamp Genomic DNA Kit (TIANGEN BIOTECH, Beijing, China) according to manufacture protocol. ken toews calgaryhttp://www.bioalpha2008.com/products/tgsjhx7833.html kent offshore