Thioltrackertm violet dye
WebThe ThiolTracker Violet stain (a registered trademark of Invitrogen) is a bright fluorescent probe that is highly reactive to thiols and can be used as a convenient and effective … WebFigure 1. The comparison in the fluorescence intensity of ThiolTrace™ Violet 500 with ThiolTrackerTM Violet (Thermo Scientific) in Jurkat cells in the presence of cell culture medium. Jurkat cells were dye loaded with ThiolTrace™ Violet 500 or ThiolTrackerTM Violet for 20 minutes in a 37 oC, 5% CO 2 incubator. The
Thioltrackertm violet dye
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WebDescription: ThiolTracker Violet dye conjugated to glutathione (GSH) Overview. Exitation ... ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTracker™ Violet can be used in estimating the cellular level of reduced glutathione. ThiolTracker™ Violet offers the following ...
WebThiolTracker™ Violet Follow these guidelines for preparing and storing ThiolTracker™ Violet dye: Prepare the ThiolTracker™ Violet dye stock solution in DMSO as described in … WebThe glutathione content ( Figure 5 A) of the viable cells was determined after 4 h ( Figure 5 B) and 24 h ( Figure 5 D) using the fluorescent dye ThiolTracker Violet, which reacts actively with ...
WebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free … WebAug 3, 2012 · The dye was then removed and media containing H 2 O 2 were added at the indicated concentrations and times. ... ThiolTrackerTM Violet conjugates to reduced (GSH) but not to oxidized glutathione, therefore ThiolTrackerTM Violet fluorescence corresponds to intracellular GSH concentration. Cells were subsequently harvested by trypsinization …
WebJul 1, 2010 · The ThiolTracker Violet stain (a registered trademark of Invitrogen) is a bright fluorescent probe that is highly reactive to thiols and can be used as a convenient and …
http://login.qa.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/cell-tissue-analysis/pdfs-posters-for-cta.par.32354.file.dat/o-076497-thiol-2008-chi-hca.pdf new cross clinicWebAssay principle ThiolTrackerTM Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays Yih-Tai Chen, Robert Aggeler, Jolene … new cross ceredigionWebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of Molecular Probes™ ThiolTracker™ Violet (glutathione detection reagent) Fisher Scientific internet services in shippensburg paWebSpectrum [ThiolTracker Violet] ThiolTracker Violet is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 526 nm, giving it a fairly large Stokes' Shift of 122nm. It can be excited using a 405 nm laser paired with a 528/45 nm bandpass filter, a configuration that can be found, for example, in the BD FACSMelody™. internet services in sheboygan wiWebWhen compared with ThiolTracker Violet (Thermo Fisher Scientific), ThiolTrace Violet 500 has 10-100 fold higher intensity in cell culture medium containing growth factors. ... internet services in sarnia ontarioWebViolet excitation/emission spectra (415/516nm maxima) are optimal for multiplexing with green and red fluorescent dyes and proteins. Easy to use: remove medium, add dye, … newcross chichesterWebApr 24, 2014 · Glutathione was stained with Thioltracker Violet (Life Technologies) and was semiquantified using FACS. Mitochondrial mass Cells were stained with the mitochondrial specific dye MitoTracker Green (Life Technologies) according to the manufacturer’s instructions and were analyzed by FACS. new cross college address