Shuttle plasmid vector
WebpBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight is 2.83 x 106 daltons. *Sequencing data from Watson (confirmed at ... WebJul 21, 2024 · Short notes : Shuttle vector : A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species.Therefore, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types. The main advantage of these vectors is they can be manipulated in E. coli and then used in a …
Shuttle plasmid vector
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WebBroad-host-range bacterial cloning vector with the pUC18/19 MCS and a tetracycline resistance gene. Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a tetracycline resistance gene. ... Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) RSF1010 RepB 4693 .. 5664 = 972 bp WebMay 23, 2024 · Plasmid pTRK-892 (Addgene) containing the erythromycin resistance gene ermC, the pWV01 replicon, and the gusA1 reporter was modified by the addition of an ampicillin resistance gene from pUC19 as well as the deletion of the gusA1 reporter to generate the base plasmid pTRKa. The base pTRKa vector was used to generate …
WebJul 29, 2014 · Strategy and method for combinatorial construction of plasmids, using a shuttle vector for A. PCC7120 as an example. (A) Four different donor plasmids harboring the devices required to build a destination vector for A. PCC7120 are shown. Each device is flanked by GC-adaptors that overlap with the GC-adaptors of the previous and following … WebThis protocol describes a simple method to construct, produce and titer recombinant CAV2 vectors. After cloning the expression cassette of the gene of interest into a shuttle plasmid, the recombinant genomic plasmid is obtained by homologous recombination in the E. coli BJ5183 bacterial strain.
WebA number of shuttle vectors based on 2μm plasmid and bacterial plasmids have been constructed which can replicate either in E.coli or yeast. Yeast plasmid vectors are of four types, yeast episomal plasmids (YEps), yeast integrative plasmids (YIps) yeast replicative plasmids (YRps) and yeast centromeric plasmids (Ycps). WebYeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces …
WebYeast episomal plasmids (YEps) are shuttle vectors. They can replicate in E. coli and also in yeast. The yeast episomal plasmids and has the following parts: 1. The 2 Micron (µm) Plasmid, 2. LEU2 Gene, and 3. The pBR 322. Part # 1. The 2 Micron (µm) Plasmid: This plasmid is found in several strains of yeast, Saccharomyces cerevisiae.
WebDiscover AdEasy adenovirus vector systems. These kits save months of work by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with pAdEasy-1 plasmid, decreasing background caused by the non-recombinant shuttle plasmid. how do you know if ps4 controller is chargingWebThe pFLAG-CMV ™-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG ® fusion proteins in mammalian cells. pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using … how do you know if radiation workedWebA shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. PubMed Article. phone book salt lake cityWebThe pAUR vector series includes six E. coli -yeast shuttle vectors, each constructed for a particular application in either Saccharomyces cercevisiae, Schizosaccharomyces, Saccharomyces pombe, or Aspergillis nidulans. These vectors include a novel drug-resistance selective marker that confers Aureobasidin A resistance to transformed yeast … how do you know if radicals are like radicalsWebMar 7, 2024 · pCM29 was chosen as starting vector for building all other reporter plasmids, since it is a stable plasmid with replicates both in E. coli and S. aureus and contains sGFP under the control of sarA ... phone book sacramento caWebA new series of yeast shuttle vectors for the recovery and identification of multiple plasmids from Saccharomyces cerevisiae.. PubMed. Frazer, LilyAnn Novak; O'Keefe, Raymond T. 2007-09-01. The availability of Saccharomyces cerevisiae yeast strains with multiple auxotrophic markers allows the stable introduction and selection of more than one yeast … phone book search freeWebShuttle plasmids. Yeast-bacteria shuttle plasmids are usually able to be maintained in both E. coli and yeast and can be episomal or integrated into the host genome. First, plasmid DNA is usually amplified in E. coli before yeast gets transformed. Different antibiotic resistance cassettes are available, and also an abundance of autotrophic ... phone book search by address