Phospholipidosis assay
Webcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a WebLC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without preincubation with a mixture of …
Phospholipidosis assay
Did you know?
WebDec 1, 2011 · Phospholipidosis is a lipid storage disorder which is recognized by excessive accumulation of phospholipids in the lysosomes within cells. Phospholipidosis can result from hereditary predisposition, which is for example the case in Niemann-Pick disease, but may also be the result of drug exposure. WebPhospholipidosis (PLD) is a lysosomal storage disorder induced by compounds, notably cationic amphiphilic drugs, which although reversible interferes with cellular …
WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. WebJun 21, 2024 · 1. Introduction. Drug-induced phospholipidosis (DIPLD) is characterized by excessive intracellular accumulation of phospholipids as a result of drug treatments [1].Since the first DIPLD case reported in 1948 [2], an increasing number of drugs were identified as PLD inducers, including antibiotics, antidepressants, antipsychotics and so …
WebFeb 1, 2006 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established NBD-PE staining assay... WebTraductions en contexte de "monitored by assay" en anglais-français avec Reverso Context : If self-injection with Improvac is suspected, reproductive physiology should be monitored by assay of testosterone or oestrogen levels (as appropriate). The risk
WebWhen studying phospholipidosis/steatosis in vitro, IONTOX uses HepG2 cells in combination with LipidTox Red, as our primary model to discriminate between phospholipidosis …
WebMar 17, 2024 · This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. This assay has … cowboy betting lineWebeCiphr ® Cardio — iPS cell-derived cardiomyocytes and MEA. Structural cardiotoxicity assay — using 3D spontaneously beating microtissues. Combined hypertrophy and cardiotoxicity assay — using 3D spontaneously beating microtissues. Cardiotox screen — … cowboy bikes logoWebPhospholipidosis (PL) is a phospholipid storage disorder, resulting in excessive accumulation of phospholipids in lysosomes of various tissue types [199, 200]. From:Progress in Medicinal Chemistry, 2011 Related terms: Amiodarone Patient Phospholipid Storage Disease Lysosome Storage Disease Niemann Pick Disease … dishwasher water hose gasketWebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high … cowboy billie boem chordsWebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a … dishwasher water hook up fittingWebCyprotex offer a number of other services including reactive metabolite detection using trapping agents, a cell stress panel, drug-induced phospholipidosis and steatosis, lysosomal trapping, hemolysis, mitochondrial toxicity (mitochondrial biogenesis assay, Glu/Gal assay, functional mitochondrial toxicity assay and the mitochondrial respiratory ... dishwasher water hose extenderWebIn this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high-throughput screening (HTS), the LOPAC library of 1280 compounds was screened using a quantitative HTS platform. A group of known phospholipidosis ... dishwasher water hose connection